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Table 2: Qualitative phytochemical analysis of Z.indica fruit extracts

Fruit extracts

Sl.no Qualitative Tests ZFHE ZFCE ZFAE ZFME ZFWE
Hager’s test

Test for Alkoloids Wagner’s test --- +++ +++ +++ ---
1
Mayer’s test
Lead acetate test
Test for Flavonoids Ferric chloride test ++ ++ +++ +++ +++
2 Alkaline test
Lead acetate test -- -- ++ ++ ++
3 Test for Phenols Ferric chloride test

Copper acetate test

Salkowski test
--- ++ +++ +++ +++
4 Test for Terpenoids Liebermann-
Burchard test
HCl test Braymer’s
Test for Tannins -- -- ++ ++ ++
5 test
Test for Fehling’s test
6 Carbohydrates Benedict’s test ++ ++ ++ ++ ++
Honey comb foam
Test for Saponin + + - - +
7 test

Test for Quinones - + + + +
8 HCl test

9 Test for Resins + + - - -
Turbid test
Glycoside test
+ + + + +
10 Test for Glycosides Keller-Kiliani Test

11 Test for Proteins + + + + +
Biuret test
Positive (+) and negative (-) signs indicate the presence and absence of phytochemical compounds respectively. More + sign indicates
number of tests conducted with presence of bioactive compounds.
Quantitative phytochemical analysis:

Total bioactive compounds present in the crude extract were analyzed by Quantitative
phytochemical analysis using High-Performance liquid chromatography (HPLC). The

amount of metabolite is proportional to the height and area of the peak in the chromatogram,
and it was calculated manually. Quantitative analysis of total bioactive compounds such as

alkaloids, phenols, flavonoids, and terpenes using HPLC is represented in chart 1 and the

amount of these compounds extracted from different solvents in terms of mg/g are depicted in
table 3. Acetone extract (ZFAE) and methanol extract (ZFME) were rich in all bioactive

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