Page 431 - e-Book

Chart 2 : DPPH Scavenging activity of fruit extracts of Zanonia indica

ABTS assay:
ABTS forms a stable radical upon oxidation of one electron. This mixture is used to

determine the total antioxidant capacity. ABTS radical is reactive towards most antioxidants

present in the crude extract and change blue color to green. The reaction is monitored
spectrophotometrically at 734nm. The absorbance data were recorded against the selected

concentrations. Graph plotted in excel, inhibition percentage on y-axis and concentrations on
x-axis is represented in chart 2. Half maximal inhibitory concentration (IC50) values for

standard (Gallic acid) and the fruit extracts were inscribed in the table (4). When extracts

were compared to the standard (1.071µg/ml) with IC50 values, ZFAE, ZFME and ZFWE
showed 43.63µg/ml, 44.36µg/ml and 138.48µg/ml respectively and showed good antioxidant

activity compared to other extracts.

Chart 3 : ABTS Scavenging activity of fruit extracts of Zanonia indica

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