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and the addition of 3ml of 0.1 mM DPPH (3.94mg of DPPH in 100ml methanol). The

solution was incubated in dark at room temperature for 15 min (Iris Hinneburg et.al).
Absorbance was measured at 517 nm using a spectrophotometer [Pruthvi et.al]. Gallic acid is

used as a standard. Reagent DPPH reagent was used as control. Percentage inhibition values
of each concentration were calculated from the absorbance of the control (Ac) and

absorbance of the sample (As) using the equation (1). IC50 values were determined using a
graphical equation.

% inhibition = [Ac – As/Ac] x100 (1)

Determination of ABTS radical scavenging activity:
The potentiality of extracts to scavenge 2,2-Azino-bis, 3-ethylbenzthiazoline- 6-

sulphonic acid (ABTS) radicals were determined by preparing Various concentrations of fruit

extracts of the sample with methanol and addition of 3ml of ABTS. The solution was

incubated in dark at room temperature for 30 min (Iris Hinneburg et.al). Absorbance was
measured at 734nm using a spectrophotometer [Pruthvi et.al]. Before the assay ABTS reagent
was prepared by 7mM of ABTS in distilled water with 2.4mM potassium persulfate kept in

dark at room temperature for 15hrs. ABTS Reagent was used as control. Gallic acid is used as
a standard. Percentage inhibition values of each concentration were calculated from the

absorbance of control (Ac) and absorbance of sample (As) using the equation (1). IC50 values

were determined using a graphical equation.
Determination of FRAP assay:

FRAP (Ferric Reducing antioxidant power) assay was done by preparing different
concentrations of fruit extracts of the sample with methanol and 2.5ml of 0.2M phosphate

buffer with 6.6 PH along with the addition of 2.5ml of potassium ferricyanide. The mixture
o
was Incubated at 50 c for 20min and then 2.5ml of 10% TCA (Trichloroacetic acid) was
added. The solution was Centrifuged at 3000rpm for 10min and 2.5ml of the upper layer was

transferred to an empty test tube. 2.5ml of distilled water and 0.5ml of 0.1% ferric chloride
were added and the absorbance was measured at 700nm in a UV visible spectrometer

[Meryem El Jemli et.al]. Gallic acid is used as a standard.

RESULTS AND DISCUSSION:

Collection and extraction of fruit sample:

Shade dried Fruit samples were powdered and extracted in Soxhlet apparatus using
different solvents such as hexane, chloroform, acetone, methanol and distilled water. The

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