Page 430 - e-Book

Figure 4: HPLC analysis of total terpenes:

HPLC chromatogram of total terpenes of Zanonia indica L. fruit extracts
Anti-Oxidant Activity:

DPPH assay:
DPPH is characterized as stable nitrogen cantered free radical, delocalized spare

electrons give violet color with methanol. When it receives hydrogen atoms from the extract

solution, it reduces its violet color to yellow. The extracts which are capable of performing
this reaction is considered as an antioxidant (Brand-Williams, W. et.al). The absorbance data

were recorded against the selected concentrations. Graph plotted in excel, inhibition
percentage on y-axis and concentrations on x-axis is represented in chart 2. Half maximal

inhibitory concentration (IC50) values for standard (Gallic acid) and the fruit extracts were

inscribed in the table (4). When extracts were compared to the standard with IC50 value
(8.048 µg/ml), ZFAE and ZFME showed 176.1µg/ml and 329.01µg/ml respectively and

showed good antioxidant activity compared to other extracts.

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