Page 432 - e-Book

Table 4: IC50 values of Gallic acid and the fruit extracts

Fruit Ic50 of Ic50 of
Extracts DPPH ABTS

Gallic acid 8.048 1.071

ZFHE 4069.3 699.46
1463.32
ZFCE 344.4

ZFAE 176.1 43.62

ZFME 329.01 44.36

ZFWE 532.66 138.48

FRAP assay:

Ferric Reducing antioxidant power assay has been carried out on the fruit extracts of
Zanonia indica. Depending on the presence of active ingredients in the extract, it has been

shown that it has a reduction potential by acquiring electrons and reduced potassium

ferricyanide (Fe3+) to potassium ferrocyanide (Fe2+). Further reacting with ferric chloride, it
formed a ferric-ferrous complex and turn the solution colorless to intense blue color. Higher

absorbance indicates a higher ferric reducing power. The fruit extracts of Zanonia showed
increased absorbance as the concentration increased. The absorbance at 700nm of specific

concentration has been depicted in table (5). The values were plotted for absorbance against
the concentration of extracts using excel is represented in chart (4). The chart clearly

visualizes the ZFAE and ZFME have antioxidant properties when compared to standard

gallic acid. Gallic acid shows the absorbance of 1.119 ZFAE shows 1.099 and ZFME shows
absorbance of 1.089 at 500 µg concentration.

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