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medicine. The fruit of Zanonia indica L. also used to cure various diseases like stomach

discomforts [Julia lalmuanpuii]. Water is filled inside the empty fruit and taken for fever,
stomach-ache and urinary problems [Lallawmkimi]. Fruit is aperient, laxative and have

cooling effect and cures asthma [kuldip et.al]. Zanonia indica L. is a Cucurbitaceae member
found growing near the river streams [DE WILDE et.al]. It is a climber with long flexible

stem, usually twines around other tree and grows with the support and hence it behaves as a
liana. Leaves of Zanonia indica L. is simple large and dark green, fruit is 8cm to 10cm long,

pendulous and stated as bandolier fruit because of its arrangement [John Lindley]. Seeds are

5-6 in number, with papery wings they are 5.5cm to 6.5cm long, 1cm wide and grayish white
in color [Renner SS et.al]. Fruits usually seen during the month of December to February.

Extraction of active ingredients and phytochemical analysis of fruit of Zanonia indica L. has

been carried out in this study and anti-oxidant properties have been investigated.

METHODOLOGY:

Collection of plant sample:

The healthy fresh whole fruits were collected from Alevooru, Udupi district Karnataka,

during the month of February 2020.

Extraction of plant sample:

Fruit samples of Z. indica were collected and brought to the laboratory, washed with tap

water and distilled water. Samples are cut into small pieces using a sterile scalpel and shade

dried for about 30 to 40 days. The dried fruit samples were mechanically powdered and
100grams of powder was extracted in a Soxhlet apparatus using successive extraction method

with 1000ml of different solvents of increasing polarity viz; hexane, chloroform, acetone,

methanol, and distilled water for 24 hours each. Solvents were separated using distillation
apparatus. All the extracts were evaporated to dryness at room temperature and stored in

airtight glass bottles and kept in the refrigerator [Madhura et.al].

Extraction Yield:

Extraction yield was analyzed by successive extraction method using solvents with increasing
polarity (hexane, chloroform, acetone, methanol and water).

Percentage yield of the plant extract was calculated using the formula (Madhura et.al).

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