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Phytochemical Evaluation And Antioxidant Activity Of Zanonia Indica L.

Fruit Extracts

S. Madhura* And H. C. Shrishail

Department of PG Studies and Research in Applied Botany, Jnanasahyadri, Kuvempu
University,

Shankaraghatta- 577451, Shivamogga District, Karnataka, India [SM, HCS].
*Email of corresponding author: madhu.my3ss@gmail.com

ABSTRACT
Traditional practice for curing diseases using locally available medicinal plants is a culture

adopted by Indians from prehistoric period. Cucurbitaceae members are also used as
medicine for various sickness, one among them is Zanonia indica L. which is selected for the

study. The aim of the investigation was to extract the phytochemicals and to examine the

anti-microbial property of the selected plant. Powder of dried fruit of Z. indica was subjected
to Soxhlet extraction using different solvents to obtain crude extracts. Hexane, chloroform,

acetone, methanol and distilled water was used for extraction. Crude extracts of these solvents
showed the presence of phenols, alkaloids, terpenoids, flavonoids, steroids, carbohydrates,

tannins, saponins, glycosides quinones, resins, and proteins in preliminary analysis.
Quantitative analysis of crude extracts using HPLC showed that the acetone and methanol

extract was rich in total alkaloids, total phenols, total flavonoids and total terpenes.

Antioxidant activity of fruit extracts of Zanonia indica using DPPH assay, ABTS radical
scavenging assay and FRAP assay revealed the presence of antioxidant property. Methanol

and acetone extract of fruit was rich in antioxidants. This study evidenced the presence of
bioactive compounds as well as its antioxidant properties and hence proves the traditional

practice of the selected plant was appropriate.

Keywords: Quantitative, High Performance liquid chromatography, Cucurbitaceae, liana

phenols, percentage yield, antioxidant, methanol extract

INTRODUCTION:

Tribal people in India practice folk medicine and utilize and the plants available in their

area. The plants at their natural habitat underwent stress and resulted in production of many
metabolites which are having immense therapeutic value and can be extracted and utilized as

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