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crude extracts containing bioactive compounds were get separated according to solvent

polarity. Each solvent extract containing different bioactive compounds was evaluated
through qualitative analysis.

Extraction Yield:

The extraction yields of different solvent extracts are measured. Among the solvents,

distilled water resulted in the highest extraction yield (8.9%), followed by methanol (3.4%),

acetone (3.2%), chloroform (2.5%), and hexane (2.4%). The physical status and percentage
yield of the extracts are as specified in table 1.

Table 1: Physical status and percentage yield of the Fruit extracts

Quantity of sample used for
%
Sl no Fruit extracts extraction Physical status Yield in grams
yield
Powder(gm) Solvent(ml)

1 ZFHE 100 1000 Light green 2.4 2.4
2 ZFCE 100 1000 Dark green 2.5 2.5
3 ZFAE 100 1000 Yellow 3.2 3.2
4 ZFME 100 1000 Yellow 3.4 3.4
5 ZFWE 100 1000 Dark brown 8.9 8.9

Qualitative phytochemical analysis:

Bioactive chemicals have a medicinal properties and are extracted using different
solvents. It can be evaluated by qualitative phytochemical analysis. Zanonia indica L. fruit

extracts showed the presence of phytochemicals such as phenols, flavonoids, terpenoids,
alkaloids, steroids, saponins, proteins, carbohydrates, tannins, quinones.

The results represented in table 2 reveals the presence of Flavonoids, carbohydrates,

proteins, and glycosides were present in all the extracts. Phenols were present in ZFAE,

ZFME and ZFWE. Alkaloids were absent in ZFWE and ZFHE. In total ZFWE, ZFAE and
ZFME contain more bioactive ingredients. These bioactive compounds contain many

pharmacological properties such as antimicrobial, antidiabetic, anti-inflammatory,
antioxidant, anticancerous and hence it is essential to analyze their specific activity

[Egamberdieva et.al].

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