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Table 5: Absorbance values of FRAP at 700nm
CONC GA ZFHE ZFCE ZFAE ZFME ZFWE
100 1.041 0.748 0.799 0.789 0.85 0.84
200 1.09 0.81 0.88 0.89 0.936 0.931
300 1.1 0.9 0.92 0.985 0.951 0.949
400 1.11 0.94 0.97 0.993 0.971 0.968
500 1.119 0.969 0.989 1.099 1.089 0.995
Chart 4: FRAP assay of fruit extracts of Zanonia indica L.
CONCLUSION: Phytochemical analysis of fruit extracts of Zanonia indica L. assured the
presence of Alkaloids, Flavonoids, terpenoids and Phenols. These were rich in acetone extract
and Methanol extract. Extracts showed the best antioxidant property in all three assays.
Among all extracts fruit acetone extract and fruit methanol extract was very active and
showed positive results for antioxidant assay.
ACKNOWLEDGMENT: The authors are thankful to the Department of PG studies and
research in Applied Botany, Kuvempu University, Jnanasahyadri, Shankaraghatta,
Shivamogga and Azyme bioscience private limited laboratory, Bengaluru for providing
facilities to conduct experimental work.
AUTHOR’S CONTRIBUTIONS: The first author designed the study, experimented,
performed the statistical analysis and wrote the first draft of the manuscript. The Second
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