Page 412 - e-Book

2.4.9 Test for proteins:

1ml extract + 500μL CuSO4 sol. + 500μL of 5% NaOH sol.  Purple violet or dark blue-
black colour indicates the positive result for protein test.

2.5 Study of Mortality:
15 larvae each were introduced in 500 ml glass beaker containing 100ml of double distilled

water with various concentrations 0.1% (1mg/ml), 0.5% (5mg/ml) and 0.8% (8mg/ml) of the
plant extract and a control was maintained with double distilled water. The analysis of each

extract were repeated five times more, and each analysis series have one control. The total

numbers of dead larvae were counted after 24h and 48h of incubation for each sample and
used for further statistical analysis (Abbott, 2009). Formula for calculating mortality

.
percentage

2.6 Probit Analysis and LC50 Calculation:

Probit analysis is a kind of regression, it analyzes binomial response variable
(Ravichandran). It is used for the detection of LC50 values in dose-response experiments

(Finny, 1952). "Lethal concentration 50%" or "median lethal concentration" in short called as
LC50.

LC50- It is a concentration of compound after a specified time of exposure at which half of a
population member is dead.

The LC50 ∝ 1/ toxicity

a. Probit analysis tool worked using dose/concentration with total values and dead values. We
used an online probit analysis tool to identify LC50 (Finny, 1952).

b. LC50 were calculated using the online tool: https://www.aatbio.com/tools/lc50-calculator

This tool worked on concentration and mortality % and outcomes in the form of values and
graphs.

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