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5. CONCLUSION:

At present environmental safety is the most important consideration. Insecticides are not

much useful to target organisms with high mortality rates, so phytochemicals are the best and
eco-friendly option. According to the above research work and statistical data, it can be

concluded that C. tamala, A. indica and P. emblica leaf extract are a good source for altering
mosquito larvae life cycle and must be explored further. The low value of LC50 shows highly

toxic effect on larvae. Future research work also includes the isolation and purification of the

Phyto compound or metabolites responsible for larvae mortality. Our work especially
signifies that double-distilled water extract of plant leaf has a high potential to kill larvae. The

highly positive result of C. tamala invites many researchers to discover the most potent
larvicidal component.

6. ACKNOWLEDGEMENT:

The authors would like thank to Mr. Vinit Singh Baghel, Department of Biotechnology, Guru

Ghasidas University, Chhattisgarh, for his valuable suggestions and reviewing first draft of
manuscript. We are also thankful to our friends and colleagues for their co-operation and

motivation.

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Ali, N. O., and El-Rabaa, F. M. (2010). Larvicidal activity of some plant extracts to larvae of

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Biswas, K., Chattopadhyay, I., Banerjee, R. K., and Bandyopadhyay, U. (2002). Biological

activities and medicinal properties of neem (Azadirachta indica). Current science,
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Calixto, J. B., Santos, A. R., Filho, V. C., and Yunes, R. A. (1998). A review of the plants of

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Chang, S. T., and Cheng, S. S. (2002). Antitermitic activity of leaf essential oils and
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