Page 723 - e-Book

CONCLUSION:

This study provides a comparative protein analysis addressing the phylogenetic relationships
and evolution of the DGAT1 protein family in 64 plant species respectively from different

families to understand the variations in protein sequences, particularly in the regions
spanning the functional domains in order to account for the preferential biosynthesis of

higher or lower amounts of fatty acids. Regions in DGAT1 including MBOAT, acyl-CoA
binding signature and the catalytic site residues were found to be very much conserved

indicating a strong functional conservation. Few indels in the MBOAT domain were

observed in 1-2 regions in some species for which a detailed experimental investigation is
needed to ascertain the functional consequences of these indels. Also the variation in number

and location of low complexity regions is of particular importance as variations in oil content

have been reported due to tandem repeat regions by some researchers in Arabidopsis thaliana
and may play a role in detailed understanding of the molecular mechanism of seed oil

accumulation, quality of seed oil and finally engineering of energy crops with higher oil
content using the DGAT1 gene as a candidate.Hydrocarbon based biofuel production mainly

depends on their biosynthetic pathways and the source of feedstock. Both play an important
role in the overall production process.TAG is one of the important precursor of biofuel

production and DGAT plays important role as a key enzyme for TAG accumulation in seeds

of angiospermic plants.
REFERENCES:

Cases S., Novak S., Zheng Y.W., Myers H.M., Lear S.R., et al. (1998) ACAT-2, second
mammalian acyl-CoA/cholesterol acyltransferase. Its cloning, expression, and

characterization. J. Biol. Chem., 273:26755–26764 [PubMed].
Chen X., Snyder C.L., Truksa M., Shah S. and Weselake R.J. (2011) sn-Glycerol-3-

phosphate acyltransferases in plants. Plant Signal. Behav. 6(11):1695–1699 [PMC free

article] [PubMed].
Chi X., Hu R., Zhang X., Chen M., Chen N., Pan L., et al. (2014) Cloning and functional

analysis of three diacylglycerol acyltransferase genes from peanut (Arachis

hypogaea L.). PLoS ONE, 9(9):e105834. doi:10.1371/journal.pone.0105834.
Darabi M. and Seddigh, S. (2013) Conserved motifs identification of 3-hydroxy-3-

methylglotaryl-coenzyme Areductase (HMGR) protein in some different species of
drosophilidae by bioinformatics tools. Ann. Biol. Res., 4(4):158–163.

http://www.missouribotanicalgarden.org/
http://www.ncbi.nlm.nih.gov

713