Page 614 - e-Book

Green tea (Ethanol extract) Black tea (Ethanol extract)

Phytochemicals Results Phytochemicals Results

Tannins + Tannins +

Saponins + Saponins +

Alkaloids - Alkaloids -

Flavonoids + Flavonoids +

Glycosides - Glycosides -

Quinone + Quinone +

Phenols + Phenols +

Terpenoids - Terpenoids +

Coumarins - Coumarins -

Anthraquinone - Anthraquinone -

Cardiac glycosides + Cardiac glycosides +

Table 2: Table showing qualitative analysis of phytochemicals in green tea and black
tea extracts.

Quantitative phytochemical analysis

Quantitative phytochemical analysis was done for tannins and flavonoids. The standard graph
of tannic acid and quercitin were plotted with the OD obtained for the different

concentrations of the two compounds. From this standard graph, it was observed that 0.5g of

green tea contains 50,744µg/ml of tannins and 183.846µg/ml of flavonoids. 0.5g of black
tea contains 34,116µg/ml of tannins and 237.942µg/ml of flavonoids.

DISCUSSION

The conventionally available antibiotics might have numerous side effects and bacteria can

become drug resistant after few days of usage. Hence, there is a need to produce antibiotics
from naturally available plants. In the present study, P. aeruginosa was more susceptible to

green tea and black tea. And E. coli was least susceptible. In previous studies done by
M. Radji et al., 2013, P. aeruginosa which is implicated in various nosocomial infections

including bacteremia and urinary tract infection is multi drug resistant bacteria, showed the

604