Page 610 - e-Book

Test for Glycosides

3ml of chloroform and 10% ammonia solution were added to 2ml of the tea extract.
Pink color shows the presence of glycosides.

Test for Quinones
To 1ml of tea extract, 1ml of concentrated sulfuric acid was added. Red color

indicates presence of quinone.

Test for Phenols
2ml of distilled water and a few drops of 10% ferric chloride were added to 1ml of tea

extract. Blue green color indicates presence of phenols.
Test of Terpenoids

2ml of chloroform and 2ml concentrated sulfuric acid were added to 0.5ml of extract.

Red brown color appearance at the interface indicates terpenoids.
Test for Cardiac Glycosides

To 0.5ml of extract, 2ml glacial acetic acid and a few drops of ferric chloride were
added. This was then under layered with 1ml of concentrated sulfuric acid. Brown

ring at the interface indicates the cardiac glycosides.

Test for Coumarins

1ml of 10% sodium hydroxide was added to 1ml of the extract. Yellow color indicates the
presence of coumarins.

Test for Anthraquinone
To 1ml of tea extract, few drops of 10% ammonia solution was added. Pink color indicates

anthraquinone.
Test for Steroids

To 1ml of tea extract, chloroform and a few drops of concentrated sulfuric acid were added.

Brown ring indicates the presence of steroids and bluish brown ring indicates the presence of
Phyto steroids. (R. Roghini et al., 2018).

Procedure for quantitative estimation of tannins

100mg of tannic acid was dissolved in 100ml of distilled water was used as the standard
solution of tannic acid. Using this standard solution, different dilutions of tannic acid were

made up to 1ml with varying amount of distilled water. To this, added 0.5ml of Folin-Denis
reagent, 1ml of sodium carbonate solution and made up to 10ml with distilled water. After

shaking well, the absorbance was taken at 760nm after 30minutes.

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