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The main objective of the study is to measure the growth parameters of plants

after treatment and to test the nutrients in the soil to find out whether the biofertilizers
enriches the soil. This study is also carried out to protect the plant from soil borne pathogens.

MATERIALS AND METHODS:
The seeds taken were Cicer arietinum (Chickpeas), Capsicum annuum (Chilli), Lablab

purpureus (Hyacinth Bean) and Vigna radiata (Mung bean) were purchased from the local
market in Singanallur, Coimbatore. All the seeds belong to Fabaceae family except Chillies

which belongs to Solanaceae family. The soil type chosen was red soil which is also collected

from the same location. The bacteria (Bacillus subtilis) and fungi (Trichoderma viride) were
purchased from the Tamil Nadu Agricultural University (TNAU), Coimbatore.

Experimental Design:

For this study, Bacillus and Trichoderma were used in three different variations i.e the
seeds were treated with Bacillus separately and the second one is treated with Trichoderma

separately and finally the third one is treating the seeds with both Bacillus and Trichoderma
in 1:1 ratio. Four different proportions i.e 1%, 2%, 3% and 4% were taken for testing the

growth parameters.
Seed Treatment:

The seeds of Chickpeas and mung bean were soaked in water overnight and the seeds

of Chilli and Lablab were taken directly. 5g of chickpea seeds, lablab seeds, mung bean seeds
and chilli seeds were treated with 1% of Bacillus using rice gruel. In the same way, these

seeds were treated with other three (2%, 3%, 4%) Bacillus proportions. Then the treated
seeds were dried for some time and planted in the soil separately. 5g of Chickpea seeds, chilli

seeds, mung bean and lablab seeds were treated with 1% of Trichoderma using rice gruel.
Then the same quantity of seeds was treated with 2% of Trichoderma using rice gruel. By

using same procedure, the other two (3%,4%) proportions were also treated. 5g of Chickpea

seeds, chilli, mung bean and lablab seeds were treated with 1% of both Bacillus and
Trichoderma in 1:1 ratio i.e 0.5% of Bacillus with 0.5% of Trichoderma using rice gruel. In

the same way, the seeds were treated with other three proportions i.e 2% (1+1), 3% (1.5+1.5),

4% (2+2) respectively. After that they were dried for some time and planted in the soil. These
seed treatments were done and planted for three repeats each.

After seed treatment, they were planted and observed for about 15 days. After 5 to 10
days, the plants which were grown in the combination of both Bacillus and Trichoderma in

1:1 ratio alone were sprayed with coconut water. Coconut water is rich in cytokinins and they

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