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RESULTS AND DISCUSSION

Alternaria solani was grown on Czapek-Dox liquid medium and dry biomass was
observed for 10 days (Table-1) more rapid growth was observed during early stages and peak

growth was observed on eighth day. The growth rate lowers after eight day on word. On
second day very less biomass was observed, on third day biomass increased by several times.

Data in the table - 2 indicates that there was large variation in the growth of
Alternaria solani on different carbon compounds. This pathogen shows maximum growth on

Lactose followed by fructose and dextrose. While the minimum growth was seen in glucose.

In absence of carbon source there is no growth.
The five nitrogen compounds studied, Data in the table - 3 indicates that there was

large variation in the growth of Alternaria solani on different nitrogen compounds. This

pathogen shows maximum growth on nickel nitrate. While cobalt nitrate and barium nitrate
had more or less similar effect on the growth. The least growth was observed in potassium

nitrate. In absence of nitrogen source in the medium resulted in lowest growth as compared to
other compound.

The leaf spot pathogen on Azadirachta indica L was recorded as Alternaria solani.
th
The growth as dry biomass was observed on 8 day of incubation period. The growth
rate of Alternaria solani in culture condition showed rapid growth during early stages and

th
peak growth was observed on the 8 day of incubation period. The great extent of variation
was observed in the growth of pathogen on different carbon containing compound, maximum

growth was recorded on lactose and minimum on glucose. Of the five-nitrogen compound
were studied, maximum biomass was observed on nickel nitrate and least biomass on

potassium nitrate.

SUMMARY
The growth and nutrition requirements of Alternaria solani was studied by growing

the organism on Czapek-Dox liquid medium by either substituting or adding different
th
compounds in the medium. The growth as dry biomass was observed on 8 day of incubation
period. The growth rate of Alternaria solani in culture condition shows rapid growth during
th
early stages and peak growth was observed on the 8 day of incubation period. The great
extent of variation was observed in the growth of pathogen on different carbon containing

compound, maximum growth was recorded on lactose and minimum on glucose. Of the five
nitrogen compounds were studied, maximum biomass was observed on nickel nitrate and

least biomass on potassium nitrate. Results clearly indicates, that Alternaria solani Respond
differently to different carbon and nitrogen compounds.

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