Page 357 - e-Book

by wind, running water, insects, field workers and implements. The disease progresses most

rapidly when alternating periods of dry and wet weather occur. Many worker reviewed
physiology and biochemistry of fungi (Stall,1958; Rajderkar,1966; sharma et.al.,1985;

Sankaran et.al.,1986; Nair and Sumaridi, 2000; Bhanumathi, 2007; Mantri, 1969; Jayraj and
Ramabadran, 1998).

MATERIAL AND METHODS

The material used and methods followed during the present investigations were as
follows:

The Czapek-Dox solid and liquid medium was used as a common medium for the
studies. The composition of media was NaNo3 - 2.00g, K2HPO4 - 1.00g, MgSO4,7H2O -

0.50g, FeSO4, 7H2O - 0.01g, Sucrose - 30g, Distilled water - 1000ml.
Azadirachta indica L leaves affected with different diseases were collected from

different locations of Nashik district. Isolation from these affected leaves was carried out on

Czapek-Dox agar medium by usual tissue incubation technique. The Petri plates were
0
incubated at room temperature (22-28 C) until good growth of organism was observed. The
colonies free from contamination were transferred on Czapek-Dox agar slant and maintained
for further studies. Eight days old culture of organism was used for biochemical studies.

The Alternaria solani was grown on the Czapek-Dox liquid medium and dry biomass

was recorded at different intervals. Substituting or adding different compounds in the
Czapek-Dox liquid medium studied the effect of carbon and nitrogen on growth. The growth

th
as dry mycelial biomass was observed on the 10 day of incubation period.
OBSERVATION TABLES:

Table 1

Growth of Alternaria solani at Various incubation periods
grown on Czapek-Dox liquid medium.

Incubation periods in days Dry Biomass ( mg )
st
1 0
nd
2 5
rd
3 64
th
4 127
th
5 176
th
6 198

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