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Procedure:

1. 5ml of the working standard solution was pipette out into a 100ml conical flask.

2. To that 10ml 4% oxalic acid was added and titrated against the dye (V1ml).

Appearance of pink colour was the end point and it persisted for few minutes. The amount of
the dye consumed is equivalent to the amount of Ascorbic acid.

3. 2gm fresh fruit material (tomato/eggplant) was crushed in 10ml 4% oxalic acid,
centrifuged it and made volume upto 100ml. From this solution 5ml was pipette out.

4. 5ml of supernatant was pipette out and 10ml 4% oxalic acid was added and titrated
against the dye.

RESULT AND DISCUSSION:
Vitamin C is the major water – soluble antioxidant within the body (Sies & Wilhelm, 1995;

Levine et al., 1986 and Levine et al., 1995). It lowers blood pressure and cholesterol level of

blood (Rath, 1993). It not only reduces the possibility of cold but also effectively prevents
secondary viral or bacterial complications. It also effectively reduces the risk of cancer

(Levine et al., 1986; Block, 1992; Feri et al., 1994; Block, 1991 and Jacobs, 1993). Ample
amount of vitamin-C is found in citrus fruits and vegetables. (Bendich, 1997). The

concentration of vitamin C in fruits varies substantially due to various factors such as species,
maturity, soil, climate, season, handling, pesticides, method of preparation and consumption.

The data available after analysis was analyzed by Student’s t-test with significance level
p=0.05.

Table 1. shows the effect of PRD, PDRD, on ascorbic acid content of L. esculentum. It was

determined that, the control shows higher ascorbic acid content as compared to all PRD and
PDRD doses.

In case of Profex recommended doses, the highest ascorbic content was found in PRD-2

(0.084 mg) and least was found in PRD-3 and PRD-4 (0.045 mg). It may due to the fact that,

nitrates and nitrite level is increase due to the application of conventional pesticides.

In case of double recommended doses, the highest content was observed in PDRD-1, PDRD-
3 and PDRD-5 (0.075 mg) and least was found in PDRD-4 (0.06 mg). According to

(Matsuura et al., 2001), Vitamin C content in plants shows variation according to soil fertility

and nutrient availability. In the present study, we regularly nurtured plants soil with cow dung

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