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et al., 2002), temperature (Toor and Savage, 2006), fettilizers (Toor et al., 2006). Very few

works on effect of pesticides on ascorbic acid content in tomatoes. By taking all this account
in consideration, we examine and evaluate the effect of insecticide (Profex super) on the

content of ascorbic acid on tomatoes.
MATERIALS AND METHODS:

Seeds:

Seeds of tomato (Lycopersicon esculentum Mill.), variety Laxmi (F1 Hybrid) manufactured
by nunhems by Syngenta is used in the present study. The variety was selected on the basis of

demand of seeds in the market of Nagpur.

Pesticides:

Profex super (Insecticide) containing Profenofos 40% + Cypermethrin 4% E.C. by Nagarjuna

group

Nursery and application of Insectide:
Firstly, seeds were sown in nursery bed and then transferred to the prepared field. The plants

were nurtured with cow dung. The different doses of pesticides were applied as foliar spray
during flowering stage of plants. Total five doses of pesticides were applied on the plants at

the interval of ten days as recommended 30ml/15litres and double the recommended doses

60ml/15litres of tap water. Then fruits were harvested and dry powder of fruits of tomatoes
were used to analyze the ascorbic acid.

Method:

ESTIMATION OF ASCORBIC ACID

(Sadasivam and Manickam, 1996):

Reagents:
a) Oxalic acid 4%

b) Dye Solution: 0.042gms Sodium bicarbonate in 10 ml distilled water + 0.052gm 2-6,

dichlorophenol Indophenol was added in it and made volume 200ml with distilled water.
c) Stock Standard solution: 100 mg (0.1gm) Ascorbic acid was added in 100 ml 4%

Oxalic acid solution in a standard flask (1mg/ml) (0.1gm in 1ml).

d) Working standard: 10ml of stock solution was diluted to 100ml with 4% oxalic
acid.

The concentration of working standard was 100µg/ml.

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