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dilecta), respiratory (Coelogyne henyi, Flickingeria nodosa, Habenaria diphylla), digestive

               (Blettia  campanulata),  reproductive  (Eulophia  flaccida),  haematologic  (Habenaria
               edgeworthii,  H.  intermedia,  H.  pectinata),  and  muscular  (Luisia  trichorrhiza)  disorders

               (Chauhan, 1990; Toh, 1994; Lee et al, 1995;  Husen & Rahman, 2003). Mannose-specific
               lectins from Cymbidium hybrid, Epipactis helleborine and Liparis ovata have been positively

               tested  against  AIDS  (Balzarini  et  al.,  1992;  De  Clercq,  1994).  Vanda  parviflora  has
               anticancerous and antiviral properties (Rastogi & Dhawan, 1990). Habenaria intermedia, H.

               edgeworthii,  and  Malaxis  wallichii  are  used  as  an  ingradient  in  Chyavanprash.  Rishbak

               (Malaxis  acuminata),  Jeevak  (Malaxis  muscifera),  Rasna  (Rhynchostylis  retusa),  Ridhi
               (Habenaria  intermedia)  and  Vridhi  (  H.  edgeworthii),  Jivanti  (Eria  muscicola),  Salem

               (Eulophia  campestris),  and  Zeuxine  strateumatica  (Shwethuli)  are  commonly  used  orchid

               drugs  in  the  Ayurvedic  and  Unani  system  of  medicine.  The  therapeutic  significance  of
               orchids has been attributed to their huge reservoir of phytochemicals (alkaloids, flavonoids,

               glycosides, carbohydrates).
               India is a rich repository of orchids. The great geographic expanse with varied climatic zones

               not only influenced the distribution and composition but also accounts for enormous orchid
               diversity in India. About 1229 orchid species in 184 genera (Karthikeyan, 2000) grow in the

               country and these include nearly 352 species which are endemic to the country (Singh, 2001).

               As many as110 species of Indian orchids are medicinally important most of which have been
               collected from their natural habitats to the point of rarity. Many stunning species have gone

               extinct  (Aphyllorchis  gollanii,  Coelogyne  treutleri,  Anoectochilus  rotundifolius,
               Paphiopedilum charlesworthii,Vanda wightii,Pleione lagenaria and Zeuxine pulchra) (Singh,

               2001).  Dactylorhiza  hatagirea  (Ved  &  Tandon,  1998)  Acanthiphippium  bicolor,
               Bulbophyllum aureum, Calanthe alpina, Eulophia candida, Habenaria andamanica, Malaxis

               acuminata,  Paphiopedilum  druryi,  Renanthera  imschootiana,  Rhynchostylis  latifolia  etc.

               (Sarkar 1995) are primarily on the list of endangered species.
               MATERIALS AND METHODS

               Himachal pradesh a hill state of north India located in lap of Himalayas with wide variation

               in altitude ,topography and climate.The state owes 500 species of medicinal plants most of
               these  are  used  by  locals  in  traditional  medicines,folk  uses  and  in  pharmaceutical

               industries.Shimla the state capital and queen of hills is located at 31.61˚N and 77.10˚E in the
               south western ranges of the Himalayas with altitude ranging from 2000-2500 m above sea

               level.Temperature  ranges  from  4℃  to  31℃  with  annual  precipitation  about  1575mm.The
               climate varies from subtropical to warm temperate.The biodiversity of medicinal orchids of




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