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Statistical Analysis
One-way analysis of variance (ANOVA) was carried out to analyze data by using SPSS
16.0 software. Means of all values were compared by Tukey’s test to detect significant
difference between treatments. Standard error was plotted in all the graphics.
RESULTS
Growth Parameters
(A
) Root length was increased by increasing PGRs concentrations in S. marianum.
Maximum root length was observed at 100 ppm concentration of IAA at all growth stages
(E)
(Fig. 1A). At 40 days of spraying, root length was increased by 58.88% at 100 ppm
concentration of IAA in comparison to control plants (p<0.05).
Leaf area increased about 93.8% at 100 ppm concentration of IAA at 40 days of spraying
in comparison to control plants (p<0.05). At 60 days of spraying, leaf area significantly
decreased at 25, 50 ppm concentrations of IAA in comparison to control plants (Fig. 1B).
Minimum leaf area (26.7%) was measured at 25 ppm concentration of IAA at 60 days of
spraying (p<0.05). At 25 ppm concentration of IAA at 60 days of spraying, leaf area
decreased in comparison to the control plants.
Number of leaves decreased about 12.87% at 100 ppm concentration of IAA at 20 days
(C
) of spraying in comparison to control plants (p>0.05). Leaf number increased with increasing
IAA concentration at 40 days of spraying (Fig. 1C). A noteworthy difference (p<0.05) was
detected at all PGRs treatments after 20, 40 and 60 days of spraying.
Maximum fresh weight among all the treatments is at 100 ppm concentration of IAA
after 60 days of spraying (Fig. 1D) which is 34.79% more in comparison to the control plants
(p<0.05). A significant difference was observed in fresh weight of the plants between 20, 40
and 60 days of spraying.
Dry weight of the plant was significantly affected by PGRs. It was enhanced with the
growth of the plant. The minimum reduction in dry weight was at the 40 days of spraying at
100 ppm concentration of IAA in comparison to control plants (p>0.05, Fig. 1E).
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