Page 254 - e-Book

against some chronic illnesses, such as heart disease and cancer. A study has been attempted

to examine the effect of different concentrations of Padina tetrastromatica SLF on
germination and growth of Pisum sativum.

Materials and Methods

Padina tetrastromatica was collected from Thikkodi beach Calicut. Seaweed was collected

with hand and washed with seawater to remove the sand particles and epiphytes.
Then it was kept in an ice box and transferred to the laboratory and washed thoroughly using

tap water to remove the salt on the surface of the sample then the seaweed were spread on

blotting paper to remove the excess water.The SLF was prepared by following method of
Thirumaran et al (2009). One kg seaweed was cut into small pieces and autoclaved for one

hour and the hot extract were filtered though a double layered cheese cloth and they allow to
cool in the room temperature. The filtrate was then centrifuged at 10,000 rpm, for 30 min at

4°C, and the resulting supernatant was taken as 100%. Seaweed extract was stored in
refrigerator for further studies.Test crop plant: - The crop plant, selected for the present study

was Pisum sativum and the seeds for the study purpose were collected from "Krishibhavan"

Chelannur. The seeds with uniform size, colour and the weight are segregated and used for
the experimental purposeA total of 210 seeds were selected to conduct the experiment. Seeds

were placed in various petridishes, each with ten seeds. Each treatment was given triplicate as
a standard procedure to minimize the error. One sample is watered regularly with distilled

water and it is considered as control (T0). Other samples were treated with 10%(T1)

20%(T2)30%(T3) 40%(T4) 50%(T5) &100%(T6).Growth and germination percentage were
recorded on fifth day, tenth& fifteenth day of experiment.

Result and Discussion

Table-1 Effect of SLF on 15 th day

Radicle Hypocotyl Seedling Germination Phytotoxicity Growth Vigour

length length index index
(cm) (cm)

- - 1.89

T0 1.22 1.14 2.36 80%
0.96

T1 0.92 0.68 1.6 60% 24.59 0.67

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