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S.No. Concentration Absorbance (nm) Total Phenolic Content

1. 1mg/ml 0.118 10.6

2. 1mg/ml 0.119 10.8

3. 1mg/ml 0.119 10.8

Mean 10.733

1. II). Total Flavonoid Content Estimation:
Standard Reading:

Sr. No. Concentration Absorbance
1. 10 0.135
2. 20 0.151

3. 30 0.165
4. 40 0.177

5. 50 0.201

Rutin

0.25

0.2

Asorbance 0.15

0.1
y = 0.0016x + 0.1184
R² = 0.9856
0.05

0
0 10 20 30 40 50 60
Concentration mg/ml

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