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50. Xanthoceras sorbifolium Sapindaceae AGO32048.1

51. Euonymus alatus Celastraceae AAV31083.1

52. Seasmum indicum Pedaliaceae XP_011093759.1
53. Vitis vinifera Vitaceae XP_002279345.1

54. Tarenaya hassleriana Cleomaceae XP_010541077.1
55. Olea europae a Oleaceae AAS01606.1

56. Perilla frutescens Lamiaceae AAG23696.1

57. Erythranthe guttata Phrymaceae EYU27329.1

58. Tetraena mongolica Zygophyllaceae AGH55993.1
59. Beta vulgaris Amaranthaceae XP_010696537.1

60. Nelumbo nucifera Nelumbonaceae XP_010242271.1

61. Musa acuminata Musaceae XP_009380458.1

62. Echium pitardii Boraginaceae ACO55635.1

63. Coffea caneophora Rubiaceae CDP03141.1

64. Morus notabilis Moraceae XP_010090076.1
RESULTS
Analysis of Insertions and Deletions (Indels)

Limited number of DGAT1 amino acid sequences has been analysed previously. However, in

the present study an attempt was made to analyse variations in plant DGAT1 protein
sequences from various plant species across different genera. The DGAT1 protein structure

consists of low complexity regions towards the N-terminal many of which code for signal
sequences particularly localisation signals for endoplasmic reticulum as well as some

important recognition motifs such as EF- hand containing CaM Binding motifs (Darabi and
Seddigh, 2013). The smallest DGAT1 protein sequence in the sequence set examined in this

analysis belongs to Hordeum vulgare consisting of 470 amino acids while the largest one

belongs to the three Prunus species consisting of 539 amino acids. The reference Arabidopsis
thaliana sequence consisted of 520 residues while the DGAT1 sequence from Jatropha

curcas was observed to be 521 amino acids long. The analysis of conserved domains was
carried out using the SMART (http://smart.embl-heidelberg.de) domain analysis server in

the normal mode Figure 1(a)–(e).

A

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