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sequence alignment from Clustal Omega server (http://www.ebi.ac.uk/Tools/msa/clustalo/)

with default parameters. Based on this multiple sequence alignment, an unrooted
phylogenetic tree was generated with MEGA 6.06 (Tamura et al., 2013) standalone

evolutionary analysis package using the neighbour-joining (NJ) method. The bootstrap
analysis was performed with 1000 replicates to test the relative support for the branches

produced by the NJ Cluster analysis. The multiple sequence alignment was further processed
to generate the flashy image with important regions marked using the ESPript3.x server

(http://espript.ibcp.fr/ESPript/cgi-bin/ESPript.cgi). To obtain information about the domain

level organisation of protein sequences one representative sequence from each group (family)
was submitted to NCBI Conserved Domain Database

(http://www.ncbi.nlm.nih.gov/Structure/cdd/wrpsb.cgi) and the domains were drawn

according to their predicted positions. The details of the sequences have been compiled in
Table 1.

Table.1. Number of DGAT 1 gene analysed in this study
Sr.No. Species Family Accesion no.

1. Helianthus annuus Asteraceae ACD67882.1

2. Vernonia galamensis Asteraceae ABV21945.1

3. Phoenix dactylifera Arecaceae XP_008806896.1
4. Elaeis guinee nsis Arecaceae XP_010924968.1

5. Brassica napus Brassicaceae AIA67019.1

6. Brassica rapa Brassicaceae XP_009112604.1

7. Brassica juncea Brassicaceae AAY40784.1
8. Arabidopsis t haliana Brassicaceae NP_179535.1
9. Arabis alpina Brassicaceae KFK40306.1

10. Camelina sativa Brassicaceae XP_010415368.1

11. Eutrema salsugineum Brassicaceae XP_006409057.1

12. Capsella rubella Brassicaceae XP_006299296.1

13. Cucumis sativus Cucurbitaceae XP_004141171.1

14. Cucumis melo Cucurbitaceae XP_008459468.1

15. Jatropha curcas Euphorbiaceae ABB84383.1

16. Ricinus communis Euphorbiaceae XP_002514132.1

17. Vernicia fordii Euphorbiaceae ABC94471.1

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