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(2019) investigated the process of in situ transesterification of Chlorella vulgaris using
H2SO4 as a catalyst and reported that high FAME yield (> 90 wt.%) could still be attained
at the moisture content lesser than 15 wt% .
Effect of methanol to oil ratio
Methanol always played an important role in trans esterification because during the trans
esterification reaction, methanol was considered not only as reactant, converting the lipids to
fatty acid alkyl esters, but also as solvent, extracting the lipids from the biomass. Previous
studies have demonstrated that methanol was optimal for biodiesel production and the
volume of methanol had a dramatic effect on biodiesel yield (Wahlen et al., 2011, Li et al.,
2011 and Georgogianni et al., 2008).
Figure 2: Effect of Volume of Alcohol Used on Biodiesel Yield
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